Luciferase Stable IMR-32 cells are derived from primary stage 4 neuroblastoma with MYCN gene amplification (a characteristic of advanced neuroblastoma). These cells have applications in understanding more about local and metastatic tumor growth patterns and exploring new cancer therapeutics.
Quantity: 1 million cells/1.0 ml
Organism: Human (H. sapiens)
Source Organ: BrainBioSafety Level: II
Growth Properties: Adherent
Morphology: Neuronal
Population Doubling: 20 hours
Seeding Density: 30,000-40,000 cells/cm2
Markers: Luciferase (gene insertion), PcDNA3-Luc2 vector, neomycin resistance
Applications: For Research Use Only
Donor Age: 13 months old
Donor Ethnicity: Not disclosed
Propagation: The base medium for this cell line is Prigrow II medium available at abm, Cat. No. TM002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (TM999)* to a final concentration of 10%, Penicillin/Streptomycin Solution (G255) to a final concentration of 1%, and 1X GlutaMAX (Gibco).Change media every 2-3 days.Carbon dioxide (CO2): 5%, Temperature: +37.0°C.* Do not use heat-inactivated FBS for cell culture unless specified otherwise.Preservation: 1. Freeze Medium: Complete growth medium with 20% FBS and 10% DMSO.2. Storage Temperature: Liquid nitrogen vapour phase.
Quality Control: 1) Western blot; 2) Bioluminescence; 3) Inner salt (MTS) assay
Shipping Conditions: Dry Ice
Storage Conditions: -180°C