Recommended Medium: It is recommended to use Fibroblast Medium (FM, Cat. no. SC2301) for culturing HDF-GFP in vitro.
Product Use: HDF-GFP are for research use only. They are not approved for human or animal use, or for application in in vitro diagnostic procedures.
Storage: Upon receiving, directly and immediately transfer the cells from dry ice to liquid nitrogen and keep the cells in liquid nitrogen until they are needed for experiments.
Shipping: Dry ice.
References: [1] Ghaleh, H. E. G., Bolandian, M., Dorostkar, R., Jafari, A., & Pour, M. F. (2020). Concise review on optimized methods in production and transduction of lentiviral vectors in order to facilitate immunotherapy and gene therapy. Biomedicine & Pharmacotherapy, 128, 110276.
[2] Kisiel, M. A., & Klar, A. S. (2019). Isolation and culture of human dermal fibroblasts. In Skin tissue engineering: methods and protocols (pp. 71-78). New York, NY: Springer New York.
[3] Stair S, Carlson KW, Shuster S, Wei ET, Stern R. (2002) “Mystixin peptides reduce hyaluronan deposition and edema formation.” Eur J Pharmacol. 450: 291-6.