GFP1-10 Stable Expressing MDCK Cell Line

GFP1-10 Stable Expressing MDCK Cell Line was established by transducing parental MDCK cells with the pCMV-GFP1-10 plasmid. By itself, GFP1-10 is a fragment containing three residues that constitute the GFP chromophore, thus is non-fluorescent. Therefore, it is valuable cell line to be used in assays in which GFP11 is fused to an epitope of a target protein to study. The fused protein may be transfected into the cell line. Upon complementation, GFP1-10 and GFP11 fluoresces and can be visualized by microscopy.

Quantity: 10⁶ cells/1.0 ml

Organism: Dog

Source Organ: Kidney

BioSafety Level: II

Growth Properties: Adherent

Morphology: Epithelial-like

Applications: For Research Use Only

Propagation: The base medium for this cell line is Prigrow VII medium available at abm, Cat. No. TM017. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (TM999)* to a final concentration of 5% and Penicillin/Streptomycin Solution (G255) to a final concentration of 1%.Change media every 2-3 days.Carbon dioxide (CO₂): 5%, Temperature: +37.0°C.* Do not use heat-inactivated FBS for cell culture unless specified otherwise.

Preservation: 1. Freeze Medium: Complete growth medium with 20% FBS and 10% DMSO.2. Storage Temperature: Liquid nitrogen vapour phase.

Shipping Conditions: Dry Ice

Storage Conditions: -180°C