These particular HeLa cells stably express the NDE1 (NudE Neurodevelopment Protein 1) protein. It is localized at the centrosome and is part of a complex that regulates dynein function. This protein is required for the correct orientation of the mitotic spindle and is heavily involved in microtubule organization and neuronal migration.High magnifications (400-1000x) are required to observe the GFP-tagged fusion due to the intracellular localization of specific subcellular structures.
Quantity: 1 million cells/1.0 ml
Organism: Human (H. sapiens)
Source Organ: Cervix
BioSafety Level: II
Growth Properties: Adherent
Morphology: Epithelial
Applications: For Research Use Only
Propagation: The base medium for this cell line is Prigrow III medium available at abm, Cat. No. TM003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (TM999)* to a final concentration of 10%, 2 mM L-glutamine (G275) and Penicillin/Streptomycin Solution (G255) to a final concentration of 1%.Change media every 2-3 days.Carbon dioxide (CO2): 5%, Temperature: +37.0°C.* Do not use heat-inactivated FBS for cell culture unless specified otherwise.
Quality Control: 1) Phenotypic analyses via microscopy
Shipping Conditions: Dry Ice
Storage Conditions: -180°C