These particular HeLa cells stably express the CENPA gene which encodes for a centromere protein. This protein is involved in targeting to the centromere. High magnifications (400-1000x) are required to observe the GFP-tagged fusion due to the intracellular localization of specific subcellular structures.Quantity: 10⁶ cells/1.0 mlOrganism: HumanSource Organ: CervixBioSafety Level: IIGrowth Properties: AdherentMorphology: EpithelialPopulation Doubling: 24-34 hoursSeeding Density: 40,000-50,000 cells/cm2Markers: CENP-A, CSAP, GFPApplications: For Research Use OnlyPropagation: The base medium for this cell line is Prigrow III medium available at abm, Cat. No. TM003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (TM999)* to a final concentration of 10%, 2 mM L-glutamine (G275) and Penicillin/Streptomycin Solution (>G255) to a final concentration of 1%.Change media every 2-3 days.Carbon dioxide (CO2): 5%, Temperature: +37.0°C.* Do not use heat-inactivated FBS for cell culture unless specified otherwise.Preservation: 1. Freeze Medium: Complete growth medium with 20% FBS and 10% DMSO.2. Storage Temperature: Liquid nitrogen vapour phase.Quality Control: 1) Phenotypic analyses via microscopyShipping Conditions: Dry IceStorage Conditions: -180°C