GFP Stably Expressing Jurkat Cell Line

GFP Stably Expressing Jurkat Cell Line

Quantity: 10⁶ cells/1.0 ml

Organism: Human

Source Organ: Peripheral Blood

BioSafety Level: II

Growth Properties: Suspension

Morphology: Round

Population Doubling: 40-50 hours

Seeding Density: Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.

Markers: Puromycin

Propagation: The base medium for this cell line is PriGrow II medium available at abm TM002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (TM999) to a final concentration of 10%, 0.8 µg/ml puromycin (G264.), and Penicillin/Streptomycin Solution (G255) to a final concentration of 1%. Carbon dioxide (CO₂): 5%, Temperature: +37.0°C.

Freeze Thaw: 1. Pre-warm complete media in a +37°C waterbath.2. Remove the cryopreserved vial from the liquid nitrogen storage tank.3. Thaw the cells quickly by placing the lower half of the vial into the +37°C water bath and remove after 60 seconds. There should still be a few ice crystals left after thawing. It is important not to over-thaw the cryovials as the presence of DMSO is toxic to the cells.4. Re-suspend the cells in the vial and transfer the cell suspension into a 15 ml sterile conical tube containing 5 ml complete media.5. Centrifuge cells at 1500rpm for 3 minutes to pellet.6. Aspirate out the media, leaving cell pellet undisturbed.7. Re-suspend pellet in fresh culture medium and plate in new culture vessel.8. Incubate cultures at +37°C, 5% CO2.

Quality Control: 1. Puromycin selection 2. FACS Sorting of very high GFP expressing population.

Shipping Conditions: Dry Ice

Storage Conditions: -180°C