AZ-GR Stable HeLa Luciferase Reporter Cell Line

The glucocorticoid receptpr (GR) is a ligand-activated transcription factor that binds endogenous glucocorticoids such as cortisol and synthetic glucocorticoids commonly used as anti-inflammatory or immunosuppressive drugs. The AZ-GR Stable HeLa Luciferase Reporter Cell Line is a selective, sensitive and reliable tool for high throughput gene reporter assays examining GR activity. It was created by stably transfecting plasmids containing glucocorticoid response elements (GREs) coupled with the luciferase reporter gene in to HeLa cells with endogenous GR. This cell line is particularly efficient for research on pre-clinical studies investigating novel glucocorticoid pharmaceuticals or for environmental applications, such as monitoring the levels of steroid hormones present in soil or water samples.

Quantity: 10⁶ cells/1.0 ml

Organism: Human

Source Organ: Cervix

BioSafety Level: II

Growth Properties: Adherent

Morphology: Epithelial-like

Seeding Density: Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.

Applications: For Research Use Only

Donor Age: 31 year old

Donor Gender: Female

Propagation: The base medium for this cell line is Prigrow III medium available at abm, Cat. No. TM003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (TM999) to final concentration of 10%, 4 mM L-glutamine (G275), 1% nonessential amino acids, 1mM sodium pyruvate (TM057), and Penicillin/Streptomycin Solution (G255) to a final concentration of 1%. Carbon dioxide (CO₂): 5%, Temperature: +37.0°C.

Quality Control: Selective growth on media containing hygromycin B (0.2 mg/ml)

Shipping Conditions: Dry Ice

Storage Conditions: -180°C