The Aryl hydrocarbon receptor (AhR) is a ligand-activated transcriptional factor that regulates genes implicated in drug metabolism, cell proliferation, immune responses, circadian rhythm and tumor promotion. Due to the numerous and varied effects that AhR activation may have on the cell, research investigating novel AhR ligands is important for drug interactions and chemically induced carcinogenesis. The AZ-AHR Stable HepG2 Luciferase Reporter Cell Line is a sophisticated and highly sensitive tool for gene reporter assays through the dose- and time-dependent induction of luciferase activity in response to various AhR ligands.Quantity: 10⁶ cells/1.0 mlOrganism: HumanSource Organ: LiverBioSafety Level: IIGrowth Properties: AdherentMorphology: Epithelial-likeSeeding Density: Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.Applications: For Research Use OnlyPropagation: The base medium for this cell line is Prigrow III medium available at abm, Cat. No. TM003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (TM999) to final concentration of 10%, 4 mM L-glutamine (G275), 1% nonessential amino acids, 1mM sodium pyruvate (TM057), and Penicillin/Streptomycin Solution (G255) to a final concentration of 1%. Carbon dioxide (CO2): 5%, Temperature: +37.0°C.Quality Control: Selective growth on media containing hygromycinShipping Conditions: Dry IceStorage Conditions: -180°C