HL-60 cells stably expressing GFP
Quantity: 1 million cells/1.0 ml
Organism: Human
Source Organ: Peripheral Blood
BioSafety Level: II
Growth Properties: Suspension
Morphology: Round
Population Doubling: 24-40 hours
Seeding Density: Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.
Markers: Puromycin
Propagation: The base medium for this cell line is Prigrow V medium available at abm (TM015). To make the completed growth medium, add the following components to the base medium with the final concentration: 20% fetal bovine serum (TM999), 25mM L-Glutamine and Penicillin/Streptomycin Solution (G255) to a final concentration of 1%. Carbon dioxide (CO2): 5%, Temperature: +37.0°C.
Freeze Thaw: 1. Pre-warm complete media in a +37°C waterbath. 2. Remove the cryopreserved vial from the liquid nitrogen storage tank. 3. Thaw the cells quickly by placing the lower half of the vial into the +37°C water bath and remove after 60 seconds. There should still be a few ice crystals left after thawing. It is important not to over-thaw the cryovials as the presence of DMSO is toxic to the cells. 4. Re-suspend the cells in the vial and transfer the cell suspension into a 15 ml sterile conical tube containing 5 ml complete media. 5. Centrifuge cells at 200x g for 3 minutes to pellet. 6. Aspirate out the media, leaving cell pellet undisturbed. 7. Re-suspend pellet in fresh culture medium and plate in new culture vessel. 8. Incubate cultures at +37°C, 5% CO2.
Quality Control: Puromycin drug selection
Shipping Conditions: Dry Ice
Storage Conditions: -180°C