Swarm rat chondrosarcoma (RCS) cells were derived from neoplasm in rat spine and is a model for chondrosarcoma tumor development. The Cas9 expressing RCS Cell Line was established through co-transfection with hCas9_P2A_mCherry_T2a_Puro and pOG44 vector. The generation of the Cas9 expressing RCS cell line allows users to generate targeted mutations in genes via transfection or lentiviral transfection with single-guide RNAs (sgRNAs). This cell line is a valuable research tool which enables users endless possibilities to design their desired cell line for the study of chondrocytes and gene function.Organism: RatSource Organ: SpineBioSafety Level: IIQuantity: 10⁶ cells/1.0 mlGrowth Properties: Suspension/AdherentMorphology: RoundMarkers: PuromycinPropagation: The base medium for this cell line is Prigrow III medium available at abm, Cat. No. TM003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (TM999) to final concentration of 10%, 3 µg/ml Puromycin (G264), and Penicillin/Streptomycin Solution (G255) to a final concentration of 1%. Carbon dioxide (CO2): 5%, Temperature: +37.0°C.Freeze Thaw:Quality Control: 1) Puromycin selection2) Western Blot analysis for Cas9 transgene expressionShipping Conditions: Dry IceStorage Conditions: -180°C