This HeLa cell line expresses Cas9 when induced by doxycycline. A PiggyBac transposon system was used to introduce Cas9. This parental cell line is applicable for work requiring human CRISPR/Cas9 knockout cells. Lack of pre-existing guide RNA enables freedom to introduce custom gRNA for desired gene targets.Organism: Human (H. sapiens)Source Organ: CervixBioSafety Level: IIQuantity: 10⁶ cells/1.0 mlGrowth Properties: AdherentMorphology: Epithelial-likeMarkers: Neomycin resistancePropagation: The base medium for this cell line is Prigrow III medium available at abm, Cat. No. TM003. To make the complete growth medium, add the following components to the base medium: tetracycline-free fetal bovine serum (TM999)* to a final concentration of 10%, Penicillin/Streptomycin Solution (G255) to a final concentration of 1%, and L-glutamine (2mM) (G275).Change media every 2-3 days.Carbon dioxide (CO2): 5%, Temperature: +37.0°C.* Do not use heat-inactivated FBS for cell culture unless specified otherwise.Freeze Thaw:Quality Control: 1) Western blot; 2) ImmunofluorescenceShipping Conditions: Dry IceStorage Conditions: -180°C