Recommended Medium: It is recommended to use neuronal medium (NM, Cat. no. 1521) for culturing RN-hb in vitro.
Product Use: RN-hb are for research use only. They are not approved for human or animal use, or for application in in vitro diagnostic procedures.
Storage: Upon receiving, directly and immediately transfer the cells from dry ice to liquid nitrogen and keep the cells in liquid nitrogen until they are needed for experiments.
Shipping: Dry ice.
References: [1] Parent A. (1996) “Neurons.” In Carpenter's Human Neuroanatomy (9th ed., pp131-198). Quebec: Williams & Wilkins.
[2] Alberts B, Bray D, Lewis J, Raff M, Roberts M, Watson JD. (1989) Molecular Biology of the Cell (2nd ed.). New York: Garland.
[3] Herculano-Houzel S, Lent R. (2005) “Isotropic fractionator: a simple, rapid method for the quantification of total cell and neuron numbers in the brain.” J Neurosci 25(10): 2518-2521.