Recommended Medium: It is recommended to use Astrocyte Medium-animal (AM-a, Cat. no. SC1831) for culturing RA-sc in vitro.
Product Use: RA-sc are for research use only. They are not approved for human or animal use, or for application in in vitro diagnostic procedures.
Storage: Upon receiving, directly and immediately transfer the cells from dry ice to liquid nitrogen and keep the cells in liquid nitrogen until they are needed for experiments.
Shipping: Dry ice.
References: [1] Oberheim N, Goldman S, Nedergaard M. (2012) “Heterogeneity of astrocytic form and function.” Methods in Mol Biol. 814: 23-45.
[2] Pineau I, Sun L, Bastien D, Lacroix S. (2010) “Astrocytes initiate inflammation in the injured Rat spinal cord by promoting the entry of neutrophils and inflammatory monocytes in an IL-1 receptor/MyD88-depdent fashion.” Brain Behav Immun. 24: 540-53.
[3] Hantaz-Ambroise D, Blondet B, Murawsky M, Rieger F. (1994) “Abnormal astrocyte differentiation and defective cellular interactions in wobbler Rat spinal cord.” J Neurocytol. 23: 179-92.
[4] Bhalala O, Pan L, Sahni V, McGuire T, Gruner K, Tourtellotte W, Kessler J. (2012) “microRNA-21 regulates astrocytic response following spinal cord injury.” J Neurosci. 32: 17935-47.