Recommended Medium: It is recommended to use Fibroblast Medium-2 (FM-2, Cat. no. SC2331) for culturing RSF in vitro.
Product Use: RSF are for research use only. They are not approved for human or animal use, or for application in in vitro diagnostic procedures.
Storage: Upon receiving, directly and immediately transfer the cells from dry ice to liquid nitrogen and keep the cells in liquid nitrogen until they are needed for experiments.
Shipping: Dry ice.
References: [1] Pezoldt J, Wiechers C, Erhard F, Rand U, Bulat T, Beckstette M, Brendolan A, Huehn J, Kalinke U, Mueller M, Strobl B, Deplancke B, Čičin-Šain L, Sitnik K. (2021). Single-cell transcriptional profiling of splenic fibroblasts reveals subsetspecific innate immune signatures in homeostasis and during viral infection. Communications Biology, 4(1), 1355.
[2] den Haan J, Mebius R, Kraal G. (2012). Stromal cells of the mouse spleen. Frontiers in Immunology, 3, 201.
[3] Sng XYX, Voigt V, Schuster IS et al. (2026) Fibroblastic reticular cells direct the initiation of T cell responses via CD44. Nature 651, 752–762.