Renal proximal tubular epithelial cells (RPTEpiC) play a crucial role in renal function. They reabsorb nearly all of the glucose and amino acids in the glomerular filtrate, while allowing other substances of no nutritional value to be excreted in the urine. They are also a major site of injury in a variety of congenital, metabolic, and inflammatory diseases. RPTEpiC can produce inflammatory mediators such as cytokines or chemokines and actively participate in acute inflammatory processes by affecting and directing leukocyte chemotaxis via the production of IL-8 [1, 2]. RPTEpiC express IL-2R alpha and MHC class II antigens during inflammation after renal transplantation or in crescentic glomerulonephritis, indicating the capacity to participate in the pathogenesis of immune renal injury [3]. To be able to study the relationship between proximal tubular cells and a variety of renal diseases, the RPTEpiC culture provides a useful in vitro model.
Recommended Medium
It is recommended to use Epithelial Cell Medium-animal (EpiCM-a, Cat. No. SC4131) for expanding RRPTEpiC in vitro.
Product Use
This product is for research use only. It is not approved for use in humans, animals, or in vitro diagnostic procedures.
Storage
Upon receiving, directly and immediately transfer the cells from dry ice to liquid nitrogen and keep the cells in liquid nitrogen until they are needed for experiments.
Shipping
Dry ice
Warranty
Cells are only warranted if ScienCell media and reagents are used and the recommended protocols are followed.
References
[1] van Kooten, C., van der Linde, X., Woltman, A. M., van Es, L. A. and Daha, M. R. (1999) Synergistic effect of interleukin-1 and CD40L on the activation of human renal tubular epithelial cells. Kidney Int 56(1):41-51.
[2]Schmouder, R. L., Strieter, R. M., Wiggins, R. C., Chensue, S. W. and Kunkel, S. L. (1992 ) In vitro and in vivo interleukin-8 production in human renal cortical epithelia. Kidney Int 41(1):191-8.
[3] Wuthrich, R. P., Glimcher, L. H., Yui, M. A., Jevnikar, A. M., Dumas, S. E. and Kelley, V. E. (1990) MHC class II, antigen presentation and tumor necrosis factor in renal tubular epithelial cells. Kidney Int 37(2):783-92.