Hepatocyte Medium-phenol red free (HM-prf) is a complete medium designed for optimal growth of normal human hepatocytes in vitro.
It is a sterile, liquid medium which contains essential and non-essential amino acids, vitamins, organic and inorganic compounds, hormones, growth factors, trace minerals and a low concentration of fetal bovine serum (5%).
The medium is bicarbonate buffered and has a pH of 7.4 when equilibrated in an incubator with an atmosphere of 5% CO2/95% air. The medium is formulated (quantitatively and qualitatively) to provide a defined and optimally balanced nutritional environment that selectively promotes growth of normal human hepatocytes in vitro. (SC5201-prf)
Components
Each bottles ofHM-prf consists of 500 ml of basal medium, 25 ml of fetal bovine serum (FBS, Cat. No. SC0025), 5 ml of hepatocyte growth supplement (HGS, Cat. No. SC5252) and 5 ml of penicillin/streptomycin solution (P/S, Cat. No. SC0503).
Product Use
HM-prf is for research use only. It is not approved for human or animal use, or for application in in vitro diagnostic procedures.
Storage
Store the basal medium at +4°C, the HGS, the FBS and the P/S solution at -20°C. Protect from light.
Shipping
Dye ice and ambient temperature.