MSC from ScienCell Research Laboratories are isolated from postnatal day 8 C57BL/6 mouse sciatic nerve. MSC are cryopreserved as primary cultures and delivered frozen. Each vial contains >5 x 105 cells in 1 ml volume. MSC are characterized by immunofluorescence with antibodies specific to S100, GFAP, and CD90. MSC are negative for mycoplasma, bacteria, yeast, and fungi. MSC are guaranteed to further expand for 5 population doublings under the conditions provided by ScienCell Research Laboratories.
Recommended Medium
It is recommended to use Schwann Cell Medium (SCM, Cat. No. SC1701) for culturing MSC in vitro.
Product Use
Mouse Schwann Cells are for research use only. They are not approved for human or animal use, or for application in in vitro diagnostic procedures.
Storage
Upon receiving, directly and immediately transfer the cells from dry ice to liquid nitrogen and keep the cells in liquid nitrogen until they are needed for experiments.
Shipping
Dry ice.
Warranty
Cells are only warranted if ScienCell media and reagents are used and the recommended protocols are followed.
References
[1] Jessen KR,Mirsky R. (1999) "Schwann cells and their precursors emerge as major regulators of nerve development."Trends Neurosci. 22:402-10.[2] Syroid DE, Maycox PR, Burrola PG, Liu N, Wen D, Lee KF, LemkeG, Kilpatrick TJ. (1996) "Cell death in the Schwann cell lineage and its regulation by neuregulin."Proc Natl Acad Sci USA.93:9229-34. [3] Rahmatullah M, Schroering A, Rothblum K, Stahl RC, UrbanB,Carey DJ. (1998) "Synergistic regulation of Schwann cells proliferation by heregulin and forskolin."Mol Cell Biol. 18:6245-5 2