MPNF from ScienCell Research Laboratories are isolated from postnatal day 8 C57BL/6 mouse sciatic nerve. MPNF are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 105 cells in 1 ml volume. MPNF are characterized by immunofluorescence with antibodies specific to vimentin, S-100, GFAP and CD90. MPNF are negative for mycoplasma, bacteria, yeast,and fungi. MPNF are guaranteed to further expand for 5 population doublings under the conditions provided by ScienCell Research Laboratories.
It is recommended to use Fibroblast Medium (FM, Cat. No. SC2301) for culturing MPNF in vitro.
Mouse Perineurial Fibroblasts from C57BL/6 are for research use only. They are not approved for human or animal use, or for application in in vitro diagnostic procedures.
Upon receiving, directly and immediately transfer the cells from dry ice to liquid nitrogen and keep the cells in liquid nitrogen until they are needed for experiments.
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