MHMeC from ScienCell Research Laboratories are isolated from postnatal day 2 or postnatal day 8 C57BL/6 mouse liver. MHMeC are cryopreserved at passage one and delivered frozen. MHMeC are characterized by immunofluorescence with antibodies specific to mesothelin. MHMeC are guaranteed to further culture under the conditions provided by ScienCell Research Laboratories; however, MHMeC are not recommended for long-term cultures due to limited expansion capacity and senescence after subculturing.
Recommended Medium: It is recommended to use Mesothelial Cell Medium (MesCM, Cat. no. 5351) for culturing MHMeC in vitro.
Product Use: MHMeC are for research use only. They are not approved for human or animal use, or for application in in vitro diagnostic procedures.
Storage: Upon receiving, directly and immediately transfer the cells from dry ice to liquid nitrogen and keep the cells in liquid nitrogen until they are needed for experiments.
Shipping: Dry ice.
References: [1] Koopmans T, Rinkevich Y. (2018) “Mesothelial to mesenchyme transition as a major developmental and pathological player in trunk organs and their cavities.” Commun Biol 170:1-14.
[2] Lua H, Asahina K. (2016) “The role of mesothelial cells in liver development, injury, and regeneration.” Gut Liver 10(2): 166-176.
[3] Li Y, Wang J, Asahina K. (2013) “Mesothelial cells give rise to hepatic stellate cells and myofibroblasts via mesothelial-mesenchymal transition in liver injury.” Proc Natl Acad Sci 110:2324-2329.
[4] Lua I, Li Y, Pappoe L, Asahina K. (2015) “Myofibroblastic conversion and regeneration of mesothelial cells in peritoneal and liver fibrosis.” Am J Pathol 185:3258-3273.