MCGC from ScienCell Research Laboratories are isolated from postnatal day 8 C57BL/6 mouse cerebellum. MCGC are cryopreserved as primary cultures and delivered frozen. Each vial contains >1x 106 cells in 1 ml volume. MCGC are characterized by immunofluorescence with antibodies specific to neurofilament, MAP2, and β-tubulin III. MCGC are negative for mycoplasma, bacteria, yeast,and fungi. MCGC are guaranteed to further culture under the conditions provided by ScienCell Research Laboratories; however, MCGC are not recommended for expanding or long-term cultures since the cells do not proliferate in culture.
Recommended Medium
It is recommended to use Neuronal Medium (NM, Cat. No. SC1521) for culturing MCGC in vitro.
Product Use
MCGC are for research use only. They are not approved for human or animal use, or for application in in vitro diagnostic procedures.
Storage
Upon receiving, directly and immediately transfer the cells from dry ice to liquid nitrogen and keep the cells in liquid nitrogen until they are needed for experiments.
Shipping
Dry ice.
Warranty
Cells are only warranted if ScienCell media and reagents are used and the recommended protocols are followed.
References
[1] Hatten ME. (1999) "Central nervous system neuronal migration."Annu. Rev. Neurosci.22, pp. 511-39.[2] Andersen BB, Korbo L,Pakkenberg B. (1992) "A quantitative study of the human cerebellum with unbiased stereological techniques."J Comp Neurol. 326:549-60. [3] Monti B,Marri L, Contestabile A. (2002) "NMDA receptor-dependent CREB activation in survival of cerebellar granule cells during in vivo and in vitro development."Eur J Neurosci. 16:1490-8.