Mesothelial cells line the surfaces of internal organs and cavities and form an epithelial monolayer. Mesothelial cells have characteristics of both epithelial cells and mesenchymal cells and can undergo mesothelial-mesenchymal transition to become myofibroblasts upon injury. Due to their intermediate phenotype, mesothelial cells express markers of both epithelial and mesenchymal cells, but also express the mesothelial cell marker mesothelin. Mouse HMeC (MHMeC) can be used to better understand the molecular mechanisms of mesothelialmesenchymal transition, which may aid in the development of treatments for liver disease.
Recommended Medium: It is recommended to use Mesothelial Cell Medium (MesCM, Cat. no. 5351) for culturing MHMeC in vitro.
Product Use: MHMeC are for research use only. They are not approved for human or animal use, or for application in in vitro diagnostic procedures.
Storage: Upon receiving, directly and immediately transfer the cells from dry ice to liquid nitrogen and keep the cells in liquid nitrogen until they are needed for experiments.
Shipping: Dry ice.
References: [1] Koopmans T, Rinkevich Y. (2018) “Mesothelial to mesenchyme transition as a major developmental and pathological player in trunk organs and their cavities.” Commun Biol 170:1-14.
[2] Lua H, Asahina K. (2016) “The role of mesothelial cells in liver development, injury, and regeneration.” Gut Liver 10(2): 166-176.
[3] Li Y, Wang J, Asahina K. (2013) “Mesothelial cells give rise to hepatic stellate cells and myofibroblasts via mesothelial-mesenchymal transition in liver injury.” Proc Natl Acad Sci 110:2324-2329.
[4] Lua I, Li Y, Pappoe L, Asahina K. (2015) “Myofibroblastic conversion and regeneration of mesothelial cells in peritoneal and liver fibrosis.” Am J Pathol 185:3258-3273.