The 3D Gel Staining Prep Kit (3D-GSP) is designed to be a companion product for ScienCell’s collagen I-based 3D culturing kits. This kit includes reagents necessary to fix resultant gels and their embedded cells, permeabilize the gel, and incubating buffers to stain the gels with antibodies of your choice. The reagents and protocol included in this kit are optimized for immunofluorescence staining of cells in collagen-based 3D gels.