Materials supplied by user: Fluorescent microplate reader (excitation 530-560/emission 580-600) 96-well plate Quality Control Data from LDH Cytotoxicity Assay of LDH positive control with concentrations ranging from 1.9 to 125 mU/mL shows a linear relationship between fluorescent intensity and LDH concentration (Figure 1). This assay is also applied to primary cells seeded at different densities with (Maximum LDH release) and without (Spontaneous LDH release) Lysis Buffer (Figure 2).
Product Use: LDH cytotoxicity assay kit (LDH) is designed for the rapid and sensitive detection of LDH to assess cytotoxicity induced by chemical compounds and to evaluate cell-mediated cytotoxicity. The kit is suitable for use with various mammalian cell types, including 3D cell models, and in various sample types, including serum, plasma, and cell lysates. This kit is for research use only and is not approved for human or animal use, or for application in clinical or in vitro diagnostic procedures.
Shipping: Dry ice.
Storage: Upon receipt, store the LDH positive control (Cat. no. 8778a), Sodium Lactate (Cat. no. 8778b) Stop Solution (Cat. no. 8778e), Cell Lysis Buffer (Cat. no. 8778f) at +4°C. Store Resazurin (Cat. no. 8778c), and Substrate Mix (Cat. no. 8778d) at -20°C.