Telomerase reverse transcriptase (TERT) is a vital enzyme that maintains telomere length, crucial for cellular immortality and genome stability. The human TERT gene (hTERT) copy number often varies in cancer cells, serving as a key marker in oncogenesis studies. Increased hTERT copy number is linked to cancer development, tumor progression, poor prognosis, and genomic instability by enhancing telomerase activity.
Quality Control: The hTERT and SCR primer sets are validated for specificity by qPCR with melt curve analysis and gel electrophoresis. Amplification efficiency is confirmed by template serial dilution (see Appendices 1 and 2).
Product Use: HTCQ is for research use only. It is not approved for human or animal use, or for application in clinical or in vitro diagnostic procedures.
Shipping and Storage: The product is shipped on dry ice. Upon receipt, store the GoldNStart TaqGreen qPCR master mix (Cat no. SCMB6018a-1) in the dark at -20°C in a manual defrost freezer, the primers (Cat no. SC9018a and 9018b) and the reference DNA sample (Cat no. SC9018d) at -20°C in a manual defrost freezer, and the nuclease-free H2O (Cat no. SC9018c) at +4°C. Once thawed, do NOT refreeze GoldNStart TaqGreen qPCR master mix (Cat no. SCMB6018a-1), and keep in the dark at +4°C or on ice at all times.