ScienCell's Human Global DNA Methylation Quantification qPCR Assay Kit (HGDM) is designed to quantify the level of human LINE1 promoter methylation. The reference DNA sample consists of a 1:1 ratio of methylated LINE1 promoter copies to non-methylated LINE1 promotor copies (both copies represent bisulfite converted promoter sequences), and serves as a reference for calculating the ratio of methylated to non-methylated LINE1 promotor of target samples. The carefully designed primers ensure: (i) high efficiency for trustworthy quantification; and (ii) negligible non-specific amplification. Each primer set has been validated by qPCR with melt curve analysis and gel electrophoresis for amplification specificity and by template serial dilution for amplification efficiency.