This product allows the use of a flexible volume of starting sample and to scale-up depending on final yield requirement. The average product length of SCWGAK is in a range between 2 kb and 100 kb, enabling all downstream applications, such as long-range copy number variations, next-generation sequencing, array CGH genotyping or qPCR analysis. The genomic DNA yields is up to 20 µg per 30 µL reaction, depending on the quality of input sample (intact cells or DNA).
Quality Control: The performance of SCWGAK is verified by amplifying genomic DNA from 1-3 intact human cells. The quality of resultant DNA was verified by qPCR. DNase activity was NOT detected by incubating each component of SCWGAK with single-stranded and double-stranded DNA at +37oC for 24 hours.
Product Use: SCWGAK is for research use only. It is not approved for human or animal use, or for application in clinical or in vitro diagnostic procedures.
Shipping and Storage: The product is shipped on dry ice. Upon receipt, store nuclease-free H2O (Cat. no. SCMB6078d) at +4oC and all other components at -20oC in a manual defrost freezer. Aliquot as needed. Avoid repeated freeze-and-thaw cycles