Quality Control: The specificity of the primer sets is validated by qPCR with melt curve analysis. The PCR products are analyzed by gel electrophoresis. The efficiency of the primer sets is validated by template serial dilution (See Appendix). The RRTLQ kit is validated using outbred CD® IGS rat samples.
Product Use: RRTLQ is for research use only. It is not approved for human or animal use, or for application in clinical or in vitro diagnostic procedures.
Shipping and Storage: The product is shipped on dry ice. Upon receipt, store the GoldNStart TaqGreen qPCR master mix (Cat. no. SCMB6018a-1) in the dark at -20°C in a manual defrost freezer, the primers (Cat. no. SC8908a and SCR8908b) at -20°C in a manual defrost freezer, and the nuclease-free H2O (Cat. no. SC8908c) at +4°C. Once thawed, do NOT refreeze GoldNStart TaqGreen qPCR master mix (Cat. no. SCMB6018a1), and keep in the dark at +4°C or on ice at all times.