Mitochondrial DNA (mtDNA) is circular, multicopy genome DNA located in mitochondrion, a cellular organelle that plays a key role in energy production of the cell. The capacity for energy production in a cell depends on both mtDNA integrity and copy number. Substantial evidence suggests that alterations in mtDNA copy number have been correlated with aging and various age-related disorders, such as cancer, diabetes and neurodegenerative diseases.
Quality Control: The specificity of the primer sets is validated by qPCR with melt curve analysis. The PCR products are analyzed by gel electrophoresis. The efficiency of the primer sets is validated by
template serial dilution.
Product Use: RRMQ is for research use only. It is not approved for human or animal use, or for application in clinical or in vitro diagnostic procedures.
Shipping and Storage: The product is shipped on dry ice. Upon receipt, store the GoldNStart TaqGreen qPCR master mix (Cat no. SCMB6018a-1) in the dark at -20°C in a manual defrost freezer, the primers (Cat no. SCR8938a and SCR8938b) at -20°C in a manual defrost freezer, and the nuclease-free H2O (Cat no. SC8938c) at +4°C. Once thawed, do NOT refreeze GoldNStart TaqGreen qPCR master mix (Cat no. SCMB6018a-1), and keep in the dark at +4°C or on ice at all times.