Mycoplasma is the smallest and self-replicating prokaryotes that are often find as contaminant of cell cultures. Mycoplasma contamination affects cell growth, morphology and metabolism.
Because of their small size, mycoplasma can pass through filters of 0.2 μm and micoplasma contamination is resistant to commonly used antibiotics and not easy to be spotted visually. Thus, it is necessary for regular screening in control of mycoplasma contamination of cell cultures.
The Mycoplasma PCR Detection Kit offers a quick and convenient detection of low level mycoplasma infection in cell cultures.
Mycoplasma PCR Detection Kit offers highly specific and sensitive detection of 200+ strains of Mycoplasmas in less than 2 hours. This bestselling kit is designed to minimize false positives, ensuring quick and reliable routine screening of cell cultures.
Kit Features
No DNA isolation needed
Directly add cell culture supernatants to PCR reaction
Rapid and Comprehensive
Detect 200+ Mycoplasma species in less than 2 hours
Highly Sensitive and Specific
Demonstrated the highest true positive and lowest false positive rates compared to competing kits
Easy-to-use
Includes ready-to-use primer mix and positive control
Less expensive per sample compared to competing kits
(Russel et al., 2020)
Out-performs Sigma and Lonza's kits
(Russel et al., 2020)
Reference:
Russell, B. J., Horiuchi, K., Velez, J. O., Goodman, C. H., & Johnson, B. W. (2020). Mycoplasma detection in a historical arbovirus repository: Commercial kit comparison and implications for improved repository management. Journal of virological methods, 276, 113769. https://doi.org/10.1016/j.jviromet.2019.113769