ScienCell’s Human Cancer Promoter Methylation qPCR Assay Kit (HCPM) is designed to accurately quantify the methylation levels of RASSF1A, GSTP1, and CDKN2A/p16 promoters using bisulfite-converted DNA. The included reference DNA sample contains a 1:1 ratio of methylated to non-methylated promoter sequences for each target gene, serving as a calibration standard for quantifying methylation levels in unknown samples. The assay features highly specific primers that distinguish between methylated and non-methylated DNA, validated through qPCR melt curve analysis, gel electrophoresis, and serial dilution to ensure amplification specificity and efficiency.
Quality Control: All primers set are validated by qPCR with melt curve analysis. The efficiency of the primer sets is validated by template serial dilution and gel electrophoresis (See Appendices 1 and 2).
Product Use:HCPM is for research use only. It is not approved for human or animal use, or for application in clinical or in vitro diagnostic procedures.
Shipping and Storage: The product is shipped on dry ice. Upon receipt, store the GoldNStart TaqGreen qPCR master mix (Cat no. SCMB6018a-1) in the dark at -20°C in a manual defrost freezer, the primers (Cat no. 9058a to f) and the reference DNA sample (Cat no. 9058h) at -20°C in a manual defrost freezer, and the nuclease-free H2O (Cat no. 9058c) at +4°C. Once thawed, do NOT refreeze GoldNStart TaqGreen qPCR master mix (Cat no. MB6018a-1), and always keep in the dark at -20°C or on ice.