Colorimetric TUNEL Apoptosis Assay
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Colorimetric TUNEL Apoptosis AssayColorimetric TUNEL Apoptosis AssayColorimetric TUNEL Apoptosis Assay

Colorimetric TUNEL Apoptosis Assay

Cell based assay

The ScienCell™ TUNEL Apoptosis Assay is used for detection of apoptosis (programmed cell death) in individual cells based on the terminal deoxynucleotidyl transferase (TdT)-mediated dUTP-biotin nick-end-labeling (TUNEL) technology. In brief, the 3’-OH end of the DNA strand breaks in apoptotic cells is labeled with biotinylated nucleotides using the enzyme TdT. Streptavidin conjugated horseradish peroxidase (HRP) is then bound to the biotinylated nucleotides, and visualized using the peroxidase substrate, 3,3’-diaminobenzidine tetrachloride (DAB). The nuclei of apoptotic cells should be observed dark blue to bluish black under light microscope. (SC8088)
Article number:SC8088
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