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Cell based Assay
Normal mammalian cells divide for a limited number of population doublings and eventually enter an arrested state in which the cells remain alive, but do not proliferate in response to mitogens, and assume a characteristic enlarged, flattened morphology. This process, termed senescence, is thought to be a tumor suppressive mechanism and underlying cause of aging. Senescence-associated β-galactosidase (SA-β-gal) is a widely used biochemical marker for assessing senescence in cultured cells. The ScienCell™ Cell Senescence Assay provide an easy-to-use method to detect SA-β-gal by staining cells with 5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside (X-gal) at pH 6.0, a pH condition that suppress lysosomal β-galactosidase activity sufficiently to ensure that non-senescent cells remain unstained. (SC8058)
Article number:SC8058
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Stock status:Special Order Item
The Cell Senescence Assays are applied to early passage and senescent ScienCell™ Human Renal Proximal Tubular Epithelial Cells (HRPTEpiCs). Data show that the senescent cells show positive SA-β-gal staining while mostearly passage cells don't.