Normal diploid cells lose telomeres with each cell cycle. Telomere length, therefore, decreases over time and may predict lifespan. Telomere shortening has negative effects on health conditions and has been linked to many health issues including aging and cancer. Accurate and consistent quantification of telomere length is important in many aspects of cell biology such as chromosomal instability, DNA repair, senescence, apoptosis, cell dysfunctions, and oncogenesis.
Quality Control: The specificity of the primer sets is validated by qPCR with melt curve analysis. The PCR products are analyzed by gel electrophoresis. The efficiency of the primer sets is validated by
template serial dilution. The telomere length of reference genomic
DNA sample is determined by qPCR standard curve method. The ARTLQ kit is validated using outbred CD® IGS rat samples.
Product Use: ARTLQ is for research use only. It is not approved for human or animal use, or for application in clinical or in vitro diagnostic procedures.
Shipping and Storage: The product is shipped on dry ice. Upon receipt, store the GoldNStart TaqGreen qPCR master
mix (Cat no. SCMB6018a-1) in the dark at -20°C in a manual defrost freezer, the primers (Cat no. SC8918a and no. R8918b) and the reference genomic DNA sample (Cat no. SCR8918d) at -20°C in a manual defrost freezer, and the nuclease-free H2O (Cat no. SC8918c) at +4°C. Once thawed, do NOT refreeze GoldNStart TaqGreen qPCR master mix (Cat no. SCMB6018a-1), and keep in the dark at +4°C or on ice at all times.