Telomeres are repetitive nucleotide elements at the ends of chromosomes that protect chromosomes from degradation and genetic information loss. Normal diploid cells lose telomeres with each cell cycle. Telomere length, therefore, decreases over time and may predict lifespan. Accurate and consistent quantification of telomere length is important in many aspects of cell biology such as chromosomal instability, DNA repair, senescence, apoptosis, cell dysfunctions and oncogenesis.
Quality Control: The specificity of the primer sets is validated by qPCR with melt curve analysis. The PCR products are analyzed by gel electrophoresis. The efficiency of the primer sets is validated by
template serial dilution. The telomere length and mtDNA copy
number of reference genomic DNA sample are determined by qPCR standard curve method.
Product Use: ARDQ is for research use only. It is not approved for human or animal use, or for application in clinical or in vitro diagnostic procedures.
Shipping and Storage: The product is shipped on dry ice. Upon receipt, store the GoldNStart TaqGreen qPCR master
mix (Cat no. SCMB6018a-1) in the dark at -20°C in a manual defrost freezer, store the primers (Cat no. SC8958a, SCR8958b and SCR8958c) and the reference genomic DNA sample (Cat no. SCR8958e) at -20°C in a manual defrost freezer, and nuclease-free H2O (Cat no. SC8958d) at +4°C. Once thawed, do NOT
refreeze GoldNStart TaqGreen qPCR master mix (Cat no. SCMB6018a-1), and keep in the dark at +4°C or on ice at all times.