Telomere shortening has negative effects on health conditions and has been linked to many health issues including aging and cancer. Accurate and consistent quantification of telomere length is important in many aspects of cell biology such as chromosomal instability, DNA repair, senescence, apoptosis, cell dysfunctions, and oncogenesis.
Quality Control:The specificity of the primer sets is validated by qPCR with melt curve analysis. The PCR products are analyzed by gel electrophoresis. The efficiency of the primer sets is validated by template serial dilution (See Appendices 1 and 2). The telomere length of reference genomic DNA sample is quantified against a reference gDNA with known telomere length. The ABTLQ kit is validated by gDNA isolated from Bovine Dermal Fibroblasts (Cat no. SCB2300).
Product Use:ABTLQ is for research use only. It is not approved for human or animal use, or for application in clinical or in vitro diagnostic procedures.
Shipping and Storage:The product is shipped on dry ice. Upon receipt, store the GoldNStart TaqGreen qPCR master mix (Cat no. SCMB6018a-1) in the dark at -20°C in a manual defrost freezer, the primers (Cat no. SC8918a and SCB8918b) and the reference genomic DNA sample (Cat no. SCB8918d) at -20°C in a manual defrost freezer, and the nuclease-free H2O (Cat no. SC8918c) at +4°C. Once thawed, do NOT refreeze GoldNStart TaqGreen qPCR master mix (Cat no. SCMB6018a-1), and keep in the dark at +4°C or on ice at all times.