Macrophages are cells differentiated from circulating bone marrow-derived monocytes. The main function of macrophages is to remove cellular debris and destroy invading pathogens. Mouse Splenic Macrophages (MSMa) reside in the spleen and play a critical role in maintaining blood homeostasis [1]. There are three distinct populations of splenic macrophages including: red pulp macrophages, marginal zone macrophages, and marginal zone metallophillic macrophages [1, 2]. MSMa perform iron processing functions and aid in the capture of microbes and viruses in the circulatory system [1, 2]. Recent studies have shown that splenic macrophages contribute to stroke pathology and autoimmune diseases [3, 4]. MSMa are an excellent model for studying macrophage functions under normal physiological and pathological conditions.
Recommended Medium
It is recommended to use Macrophage Medium (MaM, Cat. No. SC1921) for culturing MSMa in vitro.
Product Use
This product is for research use only. It is not approved for use in humans, animals, or in vitro diagnostic procedures.
Storage
Upon receiving, directly and immediately transfer the cells from dry ice to liquid nitrogen and keep the cells in liquid nitrogen until they are needed for experiments.
Shipping
Dry ice
Warranty
Cells are only warranted if ScienCell media and reagents are used and the recommended protocols are followed.
References
[1] Borges da Silva H, Fonseca R, Pereira R, Cassado Ados A, Alvarez J, D'Imperio, Lima M. (2015) "Splenic macrophage subsets and their function during blood-borne infections." Front Immunol. 6:480. [2] Gammella E, Buratti P, Cairo G, Recalcati S. (2014) "Macrophages: central regulators of iron balance." Metallomics. 6(8): 1336-1345. [3] Liu Z, Chen C Li F, Shen J, Yang Y, Leak R, Ji X, Du H, Hu X. (2015) " Splenic responses in ischemic stroke: new insights into stroke pathology. " CNS Neurosci Ther. 21(4): 320-326. [4] McGaba T, Karlsson M. (2016) "Apoptotic cell responses in the splenic marginal zone: a paradigm for immunologic reactions to apoptotic antigens with implications for autoimmunity." Immunol Rev. 269(1): 26-43.