Macrophage Medium (MaM) is a complete medium designed for optimal growth of normal bone marrow-derived macrophages in vitro. It is a sterile, liquid medium which contains essential and non-essential amino acids, vitamins, organic and inorganic compounds, hormones, growth factors, trace minerals and a low concentration of fetal bovine serum (5%). The medium is bicarbonate buffered and has a pH of 7.4 when equilibrated in an incubator with an atmosphere of 5% CO2/95% air. The medium is formulated (quantitatively and qualitatively) to provide a defined and optimally balanced nutritional environment that selectively promotes growth of normal macrophages in vitro This product is for research use only. It is not approved for use in humans, animals, or in vitro diagnostic procedures.