RNA preparation
Total RNA Human PBMCs is prepared from freshly isolated human peripheral blood mononuclear cells of single donor.
Total RNA is purified using QC validated method and has been treated with RNase-free DNase I. and dissolved in RNase free water.
Quality Control
- The cell purity for total RNA preparation was >90 %.
- RNA quality of A260/280 ratio was in the range 1.9-2.1
- RNA Integrity number (RIN) >8, was determined using an Agilent 2100 Bioanalyzer.
- Concentration of RNA was determined using Nanodrop spectrophotometer.
- cDNA synthesis was tested by RT-PCR.
Application
- cDNA synthesis
- RT-PCR and qPCR
- RNA protection assay
- Northern blot analysis
- Purification of mRNA for library construction
- PCR-select cDNA subtraction
- Microarray.
Storage
-80˚C
Shipping
Dry-ice or in ethanol solution at room temperature.