Recombinant Rat Parvalbumin produced in
Escherichia coli. The Rat Parvalbumin is purified by proprietary chromatographic techniques.
Synonyms: Pvalb, Pva, PALB1, Parvalbumin.
Formulation: The protein was lyophilized from a concentrated solution (1mg/ml) without any additives.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Purity: Greater than 90% as determined by SDS-PAGE.
Solubility: It is recommended to reconstitute the lyophilized Parvalbumin in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Stability: Lyophilized Parvalbumin although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Parvalbumin should be stored at +4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Source:Escherichia coli. Uniprot ACC#: P02625
Applications: The Parvalbumin can be used for immunoblots, absorption experiments in immunohistochemistry, radioimmunoassay and intracellular injection. For fluid-phase adsorption in immunohistochemistry we suggest the following procedure:A- Dilute 1 µl of the monoclonal antibody 235 against parvalbumin in 5 ml of the usual buffer for immunohistochemistry (final dilution 1:5'000). B- Add 1µg of the Parvalbumin to 1ml of the diluted antibody solution and mix well. C- Incubate for at least 6 hours in the cold. D- Apply to tissue-sections and incubate for 3 days. E- Complete the immunohistochemical reaction as usual (biotinylated second antibody, ABC-complex, DAB). As a result, the immunostaining should be strongly reduced or even completely prevented.
Transportation method: Shipped at Room temperature
Safety data sheet: Inquire at info@3hbiomedical.com