Macrophage Inflammatory Protein-4 Human Recombinant produced in
E. coli is a single, non-glycosylated, polypeptide chain containing 69 amino acids and having a molecular mass of 7.8 kDa. The MIP-4 is purified by proprietary chromatographic techniques.
Synonyms: Small inducible cytokine A18, CCL18, Macrophage inflammatory protein 4, MIP-4, Pulmonary and activation-regulated chemokine, CC chemokine PARC, Alternative macrophage activation-associated CC chemokine 1, AMAC-1, Dendritic cell chemokine 1, DC-CK1, chemokine (C-C motif) ligand 18, CKb7, PARC, AMAC1, DCCK1, SCYA18.
Formulation: MIP-4 protein is lyophilized from a 0.2 µm filtered concentrated (1.0mg/ml) solution in 20mM PB, pH 7.4, 100mM NaCl.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Purity: Greater than 97% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Source:Escherichia coli.Amino acid sequence: The sequence of the first five N-terminal amino acids of MIP-4 was determined and found to be Ala-Gln-Val-Gly-Thr.
Uniprot ACC number: P55774
Transportation method: Shipped at room temperature.
Stability: Lyophilized MIP-4 although stable at room temperature.erature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution MIP-4 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
Biological Activity: The Activity MIP-4 is calculated by the ability to chemoattract Human T lymphocytes at 1-10 ng/ml corresponding to a Specific Activity of 100,000-1,000,000 IU/mg.
Solubility: It is recommended to reconstitute the lyophilized MIP-4 in sterile 18M-cm H
2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
Safety data sheet: Inquire at info@3hbiomedical.com