H9-derived Cardiomyocytes (H9-CM) from ScienCell Research Laboratories are differentiated from a pluripotent stem cell line (H9) and enriched for contracting cells (https://www.youtube.com/watch?v=5eNN37_N6zw). Through the temporal modulation of canonical Wnt signaling and growth factor induction, monolayer-cultured hPSCs are differentiated to contracting cardiomyocytes under serum-free and feeder-free conditions at high efficiency. H9-CM are cryopreserved at passage one after enrichment and delivered frozen. Each vial contains >1 x 106 cells in 1 ml volume. Cells are characterized by immunofluorescence with antibodies specific to α-Actinin and Sarcomeric Tropomyosin. H9-CM are negative for mycoplasma, bacteria, yeast and fungi. H9-CM are guaranteed to further culture under the conditions provided by ScienCell Research Laboratories. H9-CM are not recommended for expanding since the cells show limited proliferation capability in culture.
Recommended Medium: It is recommended to use Cardiac Myocyte Medium (CMM, Cat. no. 6201) for the culturing of H9-CM in vitro.
Product Use: This product is for research use only. It is not approved for human or animal use, or for application in in vitro diagnostic procedures.
Storage: Upon receiving, directly and immediately transfer Cat. no. 6250 from dry ice to liquid nitrogen and keep the cells in liquid nitrogen until they are needed for experiments. Store Cat. no. 5921 at +4°C in the dark and Cat. no. 5972 at -20°C.
Shipping: Cat. no. 6250 and Cat. no. 5972 are shipped on dry ice. Cat. no. 5921 is shipped at room temperature.