HRecMEC from ScienCell Research Laboratories are isolated from human rectum. HRecMEC are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 105 cells in 1 ml volume. HRecMEC are characterized by immunofluorescence with antibodies specific to vWF/Factor VIII and CD31 (PECAM). HRecMEC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi. HRecMEC are guaranteed to further expand for 10 population doublings under the conditions provided by ScienCell Research Laboratories.
Recommended Medium: It is recommended to use Endothelial Cell Medium (ECM, Cat. no. SC1001) for culturing HRecMEC in vitro.
Product Use: HRecMEC are for research use only. They are not approved for human or animal use, or for application in in vitro diagnostic procedures.
Storage: Upon receiving, directly and immediately transfer the cells from dry ice to liquid nitrogen and keep the cells in liquid nitrogen until they are needed for experiments.
Shipping: Dry ice.
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[2]Willett C, Duda D, Czito B, Bendell J, Clack J, Jain R. (2007) “Targeted therapy in rectal cancer.” Oncology. 21(9):1055-1065.
[3] Goldis D, Sferdian M, TarTă C, Fulger L, Totolici B, NeamTu C. (2015) “Comparative analysis of microvessel density quantified through the immunohistochemistry expression of CD34 and CD105 in rectal cancer.” Rom J Morphol Embryol. 56(2): 419-424.