Endothelial cells lining the microvasculature are known to play a critical “gatekeeper” role in the inflammatory process through their ability to recruit circulating immune cells into tissues and foci of inflammation.
Human esophageal microvascular endothelial cells (HEsMEC) exhibit a strong inflammatory immune response to LPS, cytokines, and pH exposure, thus playing a critical regulatory role in esophageal inflammation.
Human Primary Esophageal Microvascular Endothelial Cells (HEMEC) are isolated from human esophageal tissue of single donor.
HEMEC are cryopreserved at passage one and delivered frozen.
Each vial contains 500 000 cells in 1 ml volume.
HEMEC are characterized by immunofluorescence with antibody specific to vWF/Factor VIII and CD31 (PECAM) and by uptake of DiI-Ac-LDL.
HEMEC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi.
HEMEC are guaranteed to further expand for 10 population doublings under the conditions provided.
Recommended Medium
It is recommended to use Endothelial Cell Medium (ECM, Cat. no. SC1001) for culturing HEMEC in vitro.
Product Use
HEMEC are for research use only. They are not approved for human or animal use, or for application in in vitro diagnostic procedures.
Storage
Upon receiving, directly and immediately transfer the cells from dry ice to liquid nitrogen and keep the cells in liquid nitrogen until they are needed for experiments.
Shipping
Dry ice.