HEEpiC are isolated from the human esophagus. HEEpiC are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 105 cells in 1 ml volume. HEEpiC are characterized by immunofluorescence with antibody specific to cytokeratin-18. HEEpiC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HEEpiC can be further cultured under the conditions provided; however, HEEpiC are not recommended for expanding or long-term cultures due to limited expansion capacity.
Recommended Medium: It is recommended to use Epithelial Cell Medium-2 (EpiCM-2, Cat. no. SC4121) for culturing HEEpiC in vitro.
Product Use: HEEpiC are for research use only. They are not approved for human or animal use, or for application in in vitro diagnostic procedures.
Storage: Upon receiving, directly and immediately transfer the cells from dry ice to liquid nitrogen and keep the cells in liquid nitrogen until they are needed for experiments.
Shipping: Dry ice.