Although lymphatic capillary endothelial cells have many properties in common with the endothelium of blood vessels, they possess distinct structural characteristics reflecting specific functions. Lymphatic capillaries firmly integrate within the interstitium, which is connected to the extracellular matrix by fine strands of elastic fibers. These capillaries have absent or poorly developed basement membranes and lack surrounding mural cells. Lymphatic endothelium typically contains numerous invaginations and cytoplasmic vesicles, as well as, characteristic overlapping intercellular junctions.
Human Dermal Lymphatic Endothelial Cells (HDLEC) are isolated from human skin. HDLEC are cryopreserved at passage 2 after purification and delivered frozen.
Each vial contains 500 000 cells in 1 ml volume.
HDLEC are characterized by immunofluorescence with antibodies specific to CD31, podoplanin and Lyve1.
HDLEC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi.
HDLEC are guaranteed to further expand for 15 population doublings under the conditions provided.
Recommended Medium
It is recommended to use Endothelial Cell Medium (ECM, Cat. No. SC1001) for culturing HDLEC in vitro.
Storage
Upon receiving, directly and immediately transfer the cells from dry ice to liquid nitrogen and keep the cells in liquid nitrogen until they are needed for experiments.
Shipping
Dry ice.
Terms and conditions
https://www.sciencellonline.com/about/terms-and-conditions.html
Credits, Refunds, & Replacements
ScienCell Does not accept any returns and refunds are only offered in the event that the customer is approved for a replacement, but we are unable to supply the item to them within a reasonable timeframe. Replacements for the product may be given to the customer and is done so at the discretion of Sciencell. The customer may be required to pay shipping and handling costs for the replacement. If you are requesting a replacement, please download the Replacement Request Form from our website and submit the filled out form to the instructed email.
Product Replacement
Customer must notify us within a week of receiving goods to be eligible for a replacement.
1. Due to the sensitive nature of our products, no refunds or exchanges will be provided.
2. If you need a replacement, please complete and return the Technical Support Request form.
3. Please note that ScienCell will only replace defective products.
4. Product replacement exclusions include but are not limited to:
(1) If the product was refrozen
(2) if any other product or protocol was used during the subculture process
(3) if ScienCell technical product specifications were not followed
Product Use
The product is for research use only. They are not approved for human or animal use, or for application in in vitro diagnostic procedures.