Cardiac fibroblasts (CF) provide structural support for cardiac myocytes and are responsible for extracellular matrix synthesis in the heart during growth and pathophysiological conditions. CF are an important cellular component of myocardial responses to injury and the source of paracrine growth factors. CF proliferation and synthesis of matrix is essential for scar formation at sites of myocardial infarction and cardiac fibrosis. CF cultures have been widely used as a model to study the cardiac matrix remodeling by physiological (exercise) and pathological (hypertension) stressors.
RabCF from ScienCell Research Laboratories are isolated from rabbit heart. RabCF are cryopreserved at passage one and delivered frozen.
Each vial contains 500 000 cells in 1 ml volume.
RabCF are characterized by immunofluorescence with antibody specific to fibronectin.
RabCF are negative for mycoplasma, bacteria, yeast, and fungi.
RabCF are guaranteed to further expand for 10 population doublings under the conditions provided by ScienCell Research Laboratories.
Recommended Medium
It is recommended to use Fibroblast Medium-2 (FM-2, Cat. no. SC2331) for culturing RabCF in vitro.
Product Use
RabCF are for research use only. They are not approved for human or animal use, or for application in in vitro diagnostic procedures.
Storage
Upon receiving, directly and immediately transfer the cells from dry ice to liquid nitrogen and keep the cells in liquid nitrogen until they are needed for experiments.
Shipping
Dry ice.