Fibroblasts are mesenchymal cells derived from the embryonic mesoderm. They have been extensively used for a wide range of cellular and molecular studies as they are one of easiest types of cells to grow in culture. Their durability also makes them amenable to a variety of manipulations ranging from studies employing gene transfection to microinjection. In general, fibroblasts secrete a non-rigid extracellular matrix which is rich in type I and/or type III collagen [1]. There is evidence showing that fibroblasts in various organs are intrinsically different [2]. In the lymphoid compartment, fibroblasts construct a unique conduit system with intriguing size exclusion, which plays a fundamental role in regulating immune response and homeostasis of tissue fluids [3].
Recommended Medium
It is recommended to use Fibroblast Medium(Cat. No. SC2301 ) for culturing MLF in vitro.
Product Use
This product is for research use only. It is not approved for use in humans, animals, or in vitro diagnostic procedures.
Storage
Upon receiving, directly and immediately transfer the cells from dry ice to liquid nitrogen and keep the cells in liquid nitrogen until they are needed for experiments.
Shipping
Dry ice
Warranty
Cells are only warranted if ScienCell media and reagents are used and the recommended protocols are followed.
References
[1] Bradley A (1987) ” Production and analysis of chimaeras”. In Robertson EJ, Teratocarcinoman and Embryonic Stem Cells: a practical Approach (pp 113-51). Oxford: IRL Press. [2] Nagy A, Gertsenstein M, Vintersten K, Behringer R (2006) ” preparing mouse embryo Fibroblasts”. Cold Spring harbor Protocols. Pdb.prot 4398.