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Cell based assay
Antioxidants are a group of free radical scavengers, including macro and micro molecules (transferrin, vitamin E, etc.) and enzymes (catalase, GSH reductase, etc.), that can protect vital cellular target against oxidative attack from free radicals or reactive oxygen species (ROS). Due to the chemical diversity and cooperative nature of antioxidants, the measurement of total antioxidant capacity (TAC) is important in monitoring the clinical status of body fluids, as well as the cumulative capability of various food supplements to counteract oxidative stress. The ScienCell™ Total Antioxidant Capacity (TAC) Assay is a cupric ion reducing antioxidant capacity (CUPRAC) spectrophotometric method, which can simultaneously measure hydrophilic and lipophilic antioxidants at physiological pH. Trolox®, a cell-permeable, water-soluble derivative of vitamin E with antioxidant properties, serves as a standard. (SC8168)
Article number:SC8168
Unit:
Quality Control: Data from ScienCell™ TAC Assayof Trolox® solutions with concentrations ranging form 0.0125 to 0.5 mM shows a linear relationship between OD450 nm and Trolox® concentration.