This colorimetric assay is based on pyruvate kinase-catalyzed formation of pyruvate, which can be further oxidized, where the formed hydrogen peroxide is catalyzed by peroxidase and reacts with 4-aminoantipyrine to form a colored product. The intensity of the colored reaction product, which exhibits maximum absorbance at 570 nm, is directly proportional to the activity of pyruvate kinase in the sample. This assay measures PK level in various types of samples, including cells, serum, plasma, and tissue.
Product Use: This product is for research purposes only and is not approved for use in animals, humans, or diagnostic procedures.
Quality Control: Diluted PK positive control is measured with the PK Assay kit after various reaction times. The linear range of detection is 0.3 to 9.6 U/ml in a 96-well plate assay.
Shipping: Dry ice.