ScienCell's Colorimetric Histone Deacetylase Activity Assay Kit (HDAC) offers a rapid and sensitive way to determine the HDAC activity in mammalian cells and tissue samples, and to screen potential inhibitor compounds. Briefly, deacetylation of the lysine residue on the substrate by HDACs generates a product which, when incubated with a developer, releases a chromophore that can be measured at an absorbance of 405 nm.
Note: Due to the intrinsic limitation of the detection method, this kit is only suitable for measuring HDAC activity of class I, IIb and IV HDACs, which include HDAC1, HDAC2, HDAC3, HDAC6, HDAC8, HDAC10, and HDAC11.
Product Use: HDAC is for research use only. It is not approved for human or animal use, or for application in clinical or in vitro diagnostic procedures.
Shipping and Storage: The product is Dry ice. Upon receipt, components #8888a and 8888e should be stored at -20°C, #8888b, 8888c, 8888d and 8888f should be stored at -80°C. Protect from light. Repeated freeze/thaw cycles should be avoided. Aliquot if necessary. If stored properly, the kit is good for up to 12 months.
Quality Control: Serially diluted HDAC positive samples are measured using this kit.